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Application of a Plasma Reactor to Modify Egg Ovalbumin for Improved Solubility
Author(s) -
Gao G.H.,
Herald T.J.,
Muino P.L.
Publication year - 2001
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2001.tb15587.x
Subject(s) - ovalbumin , solubility , differential scanning calorimetry , endothermic process , chemistry , circular dichroism , enthalpy , chromatography , polyacrylamide gel electrophoresis , analytical chemistry (journal) , crystallography , biochemistry , organic chemistry , thermodynamics , adsorption , physics , enzyme , immune system , immunology , biology
A Plasma reactor was used to change the surface structure of ovalbumin and enhance solubility. The gases used to generate the plasmas were air, argon, nitrogen and oxygen. Ovalbumin was exposed to the gases for 10, 20, 30, 60, and 120 min. Unexposed ovalbumin served as the control. Samples were examined by polyacrylamide gel electrophoresis (PAGE), spectrophotometry, fluorometry, differential scanning calorimetry, and circular dichroism. Solubility and hydrophobicity of ovalbumin increased with exposure time, whereas endothermic enthalpy, onset PAGE. No change in CD spectra resulted from the plasma treatment. The structural changes were regarded as conformational adaptability.