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Snail Species Identification by RFLP‐PCR and Designing of Species‐Specific Oligonucleotide Primers
Author(s) -
Abdulmawjood A.,
Bülte M.
Publication year - 2001
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2001.tb15203.x
Subject(s) - biology , restriction fragment length polymorphism , snail , 16s ribosomal rna , helix pomatia , polymerase chain reaction , restriction enzyme , ribosomal rna , terminal restriction fragment length polymorphism , gene , oligonucleotide , genetics , microbiology and biotechnology , ecology
To enforce labeling regulations in the authentication of snail species it might be of importance to use nonmorphological methods. Snail meat of 2 species was analyzed by restriction fragment length polymorphism (RFLP) of the genes encoding mitochondrial 12S rRNA and 16S rRNA. Digestion of the polymerase chain reaction (PCR) products of 12S rRNA with the endonucleases Afi III, Dra I and Sty I, and the 16S rRNA PCR product with the endonucleases Nsp I, Sfu I and Taq I, yielded specific banding patterns characteristic for Helix pomatia and Helix lucorum. The meat of both species could additionally be differentiated with species‐specific oligonucleotide primers based on 16S rRNA gene sequences characteristic for both species.