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Identification and Characterization of Molecular Species of Collagen in Fish Skin
Author(s) -
Yata M.,
Yoshida C.,
Fujisawa S.,
Mizuta S.,
Yoshinaka R.
Publication year - 2001
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2001.tb11325.x
Subject(s) - horse mackerel , chemistry , pepsin , ammonium sulfate precipitation , fish <actinopterygii> , mackerel , biochemistry , chromatography , biology , fishery , size exclusion chromatography , enzyme
Pepsin‐solubilized collagen (PSC) prepared from the skin of 3 fish species—common horse mackerel, yellow sea bream, and tiger puffer—were separated into 2 fractions, major and minor, by ammonium sulfate precipitation. These collagen fractions were further purified by phosphocellulose column chromatography. From the results of SDS‐PAGE, peptide mapping, and amino‐acid analysis, the purified major and minor collagens were identified to be type I and V collagens, respectively. These results suggest that type V collagen might be widely present in fish skin as a minor collagen.