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PCR‐RFLP Analysis of the Internal Transcribed Spacer (ITS) Region for Identification of 3 Clam Species
Author(s) -
Fernández A.,
García T.,
Asensio L.,
Rodríguez M.Á.,
González I.,
Hernández P.E.,
Martín. R.
Publication year - 2001
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2001.tb04617.x
Subject(s) - ruditapes , internal transcribed spacer , biology , polymerase chain reaction , restriction enzyme , restriction fragment length polymorphism , agarose gel electrophoresis , genetics , microbiology and biotechnology , dna , gene , ribosomal rna , fishery
Restriction site analysis of the internal transcribed spacer (ITS) region amplified by the polymerase chain reaction (PCR) was used for the specific identification of 3 clam species: Ruditapes decussatus (grooved carpet shell), Venerupis pullastra (pullet carpet shell), and Ruditapes philippinarum (Japanese carpet shell). PCR amplification using primers based on nucleotide sequences of Mytilus ITS regions produced a fragment of 1195 bp in R. decussatus , 1074 bp in V. pullastra , and 1188 bp inR. philippinarum. Digestion of the PCR products with endonucleases HinfI and Rsa I , followed by agarose gel electrophoresis of the digested products, yielded specific restriction profiles that enabled direct visual identification of the species analyzed.