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Rapid PCR‐RFLP Method for the Identification of Marine Fish Fillets (Seabass, Seabream, Umbrine, and Dentex)
Author(s) -
Cocolin L.,
D'Agaro E.,
Manzano M.,
Lanari D.,
Comi G.
Publication year - 2000
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2000.tb10604.x
Subject(s) - restriction fragment length polymorphism , fish <actinopterygii> , restriction enzyme , biology , marine fish , dna extraction , fishery , food science , gene , polymerase chain reaction , biochemistry
A rapid and reliable PCR‐RFLP method was optimized to identify marine fish fillets. Seabass, seabream, umbrine, and dentex were considered in the study. After DNA extraction and PCR, the 359 bp amplification products, obtained from gene encoding the cytochrome b, were subjected to restriction enzyme analysis. All the enzymes tested were not able to distinguish all the 5 species at the same time, but the combination of the results obtained from the digestion Hae III and Nla III can be used to differentiate the fish fillets considered. The method described is sensitive, rapid, and reliable, and it could be used to expose fraudulent substitutions with less valuable fish.