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Expression of Myosin Heavy Chain Isoforms in Porcine Muscles Determined by Multiplex PCR
Author(s) -
Tanabe R.,
Muroya S.,
Chikuni K.
Publication year - 1999
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1999.tb15869.x
Subject(s) - gene isoform , myosin , complementary dna , multiplex polymerase chain reaction , reverse transcription polymerase chain reaction , microbiology and biotechnology , biology , major histocompatibility complex , heavy chain , polymerase chain reaction , multiplex , reverse transcriptase , messenger rna , gene , biochemistry , genetics
A cDNA sequence of 5′‐terminal region of myosin heavy chain (MHC) isoform 2x was determined. Based on this and published sequences, the procedure of reverse transcription‐polymerase chain reaction (RT‐PCR) employing a multiplex PCR technique was developed to analyze the expression patterns of MHC isoforms in porcine muscles. All four MHC isoforms in postnatal porcine muscles could be analyzed by this method and showed different expression patterns according to the porcine muscle types. The procedure developed in this study would be very useful to elucidate the relationships between meat quality and MHC isoform composition.