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Rapid Detection of Pseudomonas in Seafoods Using Protease Indicator
Author(s) -
Jabbar H.,
Joishy K. N.
Publication year - 1999
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1999.tb15082.x
Subject(s) - pseudomonas , proteases , protease , microbiology and biotechnology , chemistry , alkaline phosphatase , enzyme , biology , biochemistry , bacteria , genetics
The effects of Pseudomonas and the proteases it synthesizes in seafoods rendering them unfit for consumption are not fully recognized. A sandwich enzyme‐linked immunosorbent assay (ELISA) was developed wherein protease produced by Pseudomonas isolated from shrimp was used as antigen, and anti‐protease IgG conjugated with alkaline phosphatase was the second antibody. Purified protease and seafood samples, naturally contaminated or artificially inoculated with Pseudomonas , were positive by ELISA. The conventional culture method took 3 days to complete, but ELISA detected Pseudomonas with in 24h. The rapidity, simplicity and efficacy of this test make it useful for implementation of HACCP systems.