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Enzymatic Hydrolysis of Milk Proteins Under Alkaline and Acidic Conditions
Author(s) -
Dzwolak W.,
Ziajka S.
Publication year - 1999
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1999.tb15049.x
Subject(s) - chemistry , casein , hydrolysis , chromatography , hydrolysate , trypsin , pepsin , whey protein , chymotrypsin , hydrolyzed protein , enzyme , food science , biochemistry
The pH‐stat and osmometric methods were adopted for control of hydrolysis of casein and whey proteins by chymotrypsin, trypsin and pepsin. Experiments in alkaline medium showed that the mean pK values determined for amino groups in milk protein hydrolysates at 52°C were 7.11 for casein hydrolysates and 7.18 for whey protein hydrolysates. During hydrolysis in acidic medium the osmotic coefficients determined for casein and whey proteins enabled calculation of the calibration factor for the osmometer (1.05) which could be assumed as constant. Results will enable monitoring of DH during hydrolysis of milk proteins in such systems.