Premium
Identification of Flatfish Species Using Polymerase Chain Reaction (PCR) Amplification and Restriction Analysis of the Cytochrome b Gene
Author(s) -
CÉSPEDES ANA,
GARCÍA TERESA,
CARRERA ESTHER,
GONZÁLEZ ISABEL,
SANZ BERNABÉ,
HERNÁZ PABLO E.,
MARTÍN ROSARIO
Publication year - 1998
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1998.tb15710.x
Subject(s) - flatfish , platichthys , polymerase chain reaction , biology , cytochrome b , pleuronectes , agarose gel electrophoresis , microbiology and biotechnology , taqman , amplicon , restriction enzyme , primer (cosmetics) , gene , flounder , genetics , mitochondrial dna , fish <actinopterygii> , fishery , chemistry , organic chemistry
Restriction site analysis of PCR products from a conserved region of the cytochrome b gene has been used for the specific identification of sole ( Solea solea ), European plaice ( Pleuronectes platessa ) and flounder ( Platichthys flesus). Polymerase chain reaction (PCR) amplification of the cytochrome b gene using universal primers produced a 359 bp fragment in all species analyzed. Digestion of the PCR products with Nci I, Sau 3AI and Hinf I endonucleases, followed by agarose gel electrophoresis of the digested PCR products, yielded specific profiles that enabled direct identification of the fish species. This methodology should prove useful for enforcing labeling regulations in the authentication of flatfish species.