Premium
Simple, Sensitive Assay for Microbial Aminopeptidase
Author(s) -
SANZ YOLANDA,
TOLDRÁ FIDEL,
VILA RAFAEL
Publication year - 1997
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1997.tb04436.x
Subject(s) - valine , aminopeptidase , fluorometer , phenylalanine , leucine , chromatography , chemistry , biochemistry , substrate (aquarium) , fluorescence , tyrosine , alanine , biology , amino acid , ecology , physics , quantum mechanics
A fluorimetric assay has been developed using several aminoacyl‐7‐amido‐4‐methyl coumarin (AMC) substrates (L‐leucine‐, L‐alanine‐, L‐arginine‐, L‐tyrosine‐, L‐phenylalanine‐, L‐serine‐ and L‐valine‐AMC) in black multi‐well plates to measure microbial aminopeptidase activity in lactobacilli intact cell suspensions (10 9 –10 10 CFU/mL). The fluorescence emission was visually evaluated by panelists and showed that substrate concentrations of 0.01 mM were optimal for visual detection and thus useful for rapid screening of many strains. The different ratios of activities quantitated in a multiscan fluorimeter showed a potential for differentiation among strains.