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Improving Detection of Vibrio vulnificus in Octopus variabilis by PCR
Author(s) -
LEE JEE YEON,
EUN JONG BANG,
CHOI SANG HO
Publication year - 1997
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1997.tb04395.x
Subject(s) - vibrio vulnificus , octopus (software) , bacteria , brain heart infusion , microbiology and biotechnology , biology , dna extraction , pathogenic bacteria , vibrio , polymerase chain reaction , chromatography , extraction (chemistry) , chemistry , gene , biochemistry , agar , genetics , computational chemistry
PCR methods can detect foodborne pathogenic bacteria with simplicity, specificity and speed. In order to improve sensitivity and speed of PCR methods for detection of Vibrio vulnificus in small octopus homogenate, several media and culture conditions were compared. Modified brain heart infusion media containing 2% NaCl and adjusted to pH 8.0 and 30°C was most effective for enrichment of the bacteria. Procedures affecting the efficiency of template DNA extraction and target DNA amplification were also optimized. By these combined efforts, a PCR procedure capable of detecting V. vulnificus as low as 10 cells/mL within 10h was developed.