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Microbial Transglutaminase Estimation in Enzyme‐treated Surimi‐Based Products by Enzyme Immunosorbent Assay
Author(s) -
OHTSUKA T.,
SEGURO K.,
MOTOKI M.
Publication year - 1996
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1996.tb14730.x
Subject(s) - polyclonal antibodies , chemistry , tissue transglutaminase , chromatography , enzyme , monoclonal antibody , detection limit , biochemistry , food science , antibody , biology , immunology
An enzyme‐linked immunosorbent assay (ELISA) for specific determination of microbial transglutaminase (MTGase) has been developed using its polyclonal and monoclonal antibodies. In the assay we calibrated sodium dodecylsulfate (SDS)‐denatured MTGase for standard, and the detection limit of the SDS‐denatured MTGase was 0.1 μg/mL. When products were extracted with SDS and 2‐mercaptoethanol, which assured optimum protein recovery, the assay covered the expected levels for determination of MTGase in surimi‐based products. In model kamaboko products, recoveries were 50–71%. When the procedures were applied to commercial products, 0.01–0.18% (w/w) of MTGase were found in the products.