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Attachment of Salmonella typhimurium to Poultry Skin as Related to Cell Viability
Author(s) -
KIM K.Y.,
LILLARD H.S.,
FRANK J.F.,
CRAVEN S.E.
Publication year - 1996
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1996.tb14211.x
Subject(s) - viability assay , salmonella , irradiation , cell , microbiology and biotechnology , brain heart infusion , bacteria , formaldehyde , chemistry , biophysics , biology , biochemistry , genetics , physics , nuclear physics , agar
3 H‐labeled S. typhimurium cells inactivated by gamma‐radiation ( 60 Co) and by formaldehyde (5%) were used to determine if cell viability affected attachment to poultry skin. Both gamma‐irradiated and formal‐dehyde‐treated cells attached like live cells. There was no difference in attachment between cells grown in a chemically defined medium (SCDM) or in brain heart infusion broth (BHI). Live and gamma‐irradiated cells and BHI‐grown and SCDM‐grown cells had similar surface hydrophobicity. Live and gamma‐irradiated cells had similar cell surface charges, whereas BHI and SCDM‐grown cells had different surface charges. Differences in cell surface charge did not affect attachment rate.