Premium
Preliminary Studies on SDS‐PAGE and Isoelectric Focusing Identification of Sturgeon Sources of Caviar
Author(s) -
CHEN I.C.,
CHAPMAN F.A.,
WEI C.I.,
O'KEEFE S.F.
Publication year - 1996
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1996.tb13150.x
Subject(s) - isoelectric focusing , sturgeon , polyacrylamide gel electrophoresis , sodium dodecyl sulfate , chromatography , chemistry , gel electrophoresis , species identification , sodium , biology , biochemistry , fishery , zoology , fish <actinopterygii> , enzyme , organic chemistry
Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and isoelectric focusing (IEF) analyses of caviar were used to identify species of American, European and Asian sturgeon. Caviar samples of American and Gulf of Mexico sturgeon showed distinctive protein banding patterns on SDS‐PAGE. IEF gels containing 20% pH 3‐10 and 80% pH 4‐6.5 ampholyte enabled differentiation of sturgeon species. IEF was more reliable than SDS‐PAGE for identification of sturgeon species.