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HPLC Method for Cyst(e)ine and Methionine in Infant Formulas
Author(s) -
TORAN A. ALEGRÍA,
BARBERÁ R.,
FARRÉ R.,
LAGARDA M.J.,
LÓPEZ J.C.
Publication year - 1996
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1996.tb10946.x
Subject(s) - methionine , cysteic acid , derivatization , chromatography , performic acid , high performance liquid chromatography , chemistry , hydrolysis , acid hydrolysis , resolution (logic) , column chromatography , amino acid , organic chemistry , biochemistry , cysteine , cystine , artificial intelligence , computer science , enzyme
Cyst(e)ine and methionine were converted into cysteic acid and methionine sulfone by oxidizing with performic acid. The oxidized samples were then subjected to acid hydrolysis (6N HCl, 105–110°C/24 hr). After derivatization with phenylisothiocyanate, reverse phase HPLC separation was carried out at 48°C and with UV detection. Different gradients and pH values in the eluent were assayed to determine the best resolution. Analytical parameters, detection and quantification limits, linearity, precision and accuracy, were determined. The method was reliable and accurate for measuring cyst(e)ine and methionine in infant formulae.