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Microbial Biosensor System for Rapid Determination of Vitamin B 6
Author(s) -
ENDO HIDEAKI,
KAMATA ATSUSHI,
HOSHI MASAKAZU,
HAYASHI TETSUHITO,
WATANABE ETSUO
Publication year - 1995
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1995.tb09825.x
Subject(s) - clark electrode , chemistry , biosensor , membrane , chromatography , electrolyte , electrode , biochemistry
A microbial biosensor system was based on an immobilized microorganism ( Succharomyces uvarum 4288 ATCC9080) and Clark‐type oxygen electrode consisting of a platinum cathode, lead anode, alkaline electrolyte, and an oxygen permeable Teflon membrane. The determination was based on the respiratory activity of the microorganism in presence of vitamin B 6 . Optimum conditions were: concentration of immobilized cells on membrane: OD 660 , = 0.05, temperature: 30°C. When oxygen permeable Teflon membranes (0.5 mil and 2.0 mil) were employed, relationships between vitamin B 6 , (pyridoxine) and current decrease were linear in the range of 0.5 ng/mL‐2.5 ng/mL and 2.5 ng/mL‐12.5 ng/mL, respectively. One assay could be completed within 15 min. This system provided rapid and simple determinations of vitamin B 6 in marine products with good correlation to traditional microbial assay values.