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Cloning and Expression of a Limonene Degradation Pathway from Bacillus stearothermophilus in Escherichia coli
Author(s) -
CHANG HAE CHOON,
GAGE DOUGLAS A.,
ORIEL PATRICK J.
Publication year - 1995
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1995.tb09824.x
Subject(s) - escherichia coli , limonene , bioproduction , catabolism , biochemistry , chemistry , cloning (programming) , incubation , biology , industrial microbiology , microbiology and biotechnology , food science , gene , enzyme , fermentation , computer science , essential oil , programming language
The pathway encoding limonene catabolism was cloned as a 9.6kb chromosomal fragment from Bacillus stearothermophilus strain BR388 into Escherichia coli . It conferred growth on limonene as a sole carbon source and bioproduction of α‐terpineol, perillyl alcohol, and perillyl aldehyde. Incubation of the recombinant with perillyl alcohol resulted in formation of perillyl aldehyde and perillic acid. Catabolic pathways apparently affected the microbial toxicity of limonene.