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Biosensors for Analysis of Ethanol in Foods
Author(s) -
BOUJTITA M.,
MURR N.
Publication year - 1995
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1995.tb05637.x
Subject(s) - amperometry , alcohol dehydrogenase , bovine serum albumin , chemistry , ethanol , biosensor , carbon paste electrode , electrode , chromatography , electrochemistry , nad+ kinase , biochemistry , enzyme , cyclic voltammetry
An amperometric ethanol sensor was constructed by co‐immobilization of alcohol dehydrogenase (ADH) and bovine serum albumin (BSA) in carbon paste containing entrapped NAD + . Conditions for optimum electrode response were determined by manipulation of experimental variables. Incorporation of both ADH and NAD + in carbon paste resulted in better relationships between the enzymatic reaction and the electrochemical process. The surface of such an electrode could be renewed by polishing and smoothing on a clean paper. The sensor showed rapid response time and high sensitivity.