Premium
Tetrathiafulvalene‐mediated Biosensor for L‐lactate in Dairy Products
Author(s) -
MULCHANDANI ASHOK,
BASSI AMARJEET S.,
NGUYEN ANDREW
Publication year - 1995
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1995.tb05610.x
Subject(s) - biosensor , chemistry , amperometry , tetrathiafulvalene , adsorption , detection limit , chromatography , immobilized enzyme , nuclear chemistry , electrode , enzyme , biochemistry , organic chemistry , electrochemistry , molecule
A tetrathiafulvalene (TTF)‐mediated, amperometric biosensor was developed for the specific determination of L‐lactate. L‐lactate oxidase was immobilized by adsorption and retained on the surface of a carbon‐paste electrode using an electro‐polymerized film of 1,3‐phenylenediamine‐resorcinol. The response of the biosensor was maximum at pH 7.0 and 30°C. Response was linear up to 0.6 mM lactate and had a minimum detection limit of 56 PM. Response time was less than 90 sec. When stored at 4°C response was stable for > 36 days and subsequently decreased. The sensor was unresponsive after 96 days, This enzyme‐based biosensor was suitable for determining lactate concentrations in milk and yogurt.