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Quantification of Factors Which Influence Nisin's Inhibition of CIostridium botulinum 56A in a Model Food System
Author(s) -
ROGERS ANN M.,
MONTVILLE THOMAS J.
Publication year - 1994
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1994.tb05589.x
Subject(s) - nisin , starch , phospholipid , yeast extract , food science , spore , chemistry , yeast , microbiology and biotechnology , biochemistry , biology , organic chemistry , fermentation , antimicrobial , membrane
We modeled nisin's anticlostridial activity and assessed the antagonistic or potentiating influences of food ingredients. The model systems contained yeast extract, proteose peptone, and glucose; were supplemented with protein (0.075, 0.75, 7.5% w/v), phospholipid (0.075, 0.75, 7.5% w/v), or soluble starch (5, 17.5, 30% w/v); and were adjusted to pH 5.5, 6.0, or 6.5. Samples inoculated with 10 4 /mL spores were incubated at 15, 25, or 35°C. Statistical analysis developed an equation (r 2 = 0.76) that modeled the response and identified temperature as the most significant (α 0.001) variable. Nisin lost effectiveness with increasing temperature. Nisin concentration had significant positive and phospholipid negative, linear effects. Many interactive effects were significant (α 0.20). Nisin inhibited C. botulinum until its residual level dropped below a threshold, which decreased from 154 IU/mL at 35°C to 12 IU/mL at 15°C.