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A simple and rapid antibody‐capture ELISA for the detection of staphylococcal enterotoxin A in food including a simple extraction step
Author(s) -
OLIVEIRA TEREZA CRISTINA R.M.,
LEE HEATHER A.,
WYATT GARY,
HIROOKA ELISA Y.,
MORGAN MICHAEL R.A.
Publication year - 1994
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.1994.tb02099.x
Subject(s) - enterotoxin , detection limit , toxin , polyethylene glycol , chemistry , antibody , chromatography , staphylococcus aureus , microbiology and biotechnology , extraction (chemistry) , biology , escherichia coli , bacteria , immunology , biochemistry , genetics , gene
A sensitive and precise ELISA for the detection of staphylococcal enterotoxin A in food has been developed, using specific IgG anti‐toxin antibodies purified from immunized rabbits. an antibody capture format was found to be the most useful following cross‐reaction and sensitivity studies. the ELISA was used to investigate concentration of staphylococcal enterotoxin A from food samples by polyethylene glycol dialysis. the procedure was shown to be inefficient and highly variable, as well as time‐consuming. Direct analysis of aqueous food extracts without a concentration step was found to be possible. With an assay time of 140 min, the ELISA detection limit was 12.5 pg of toxin (corresponding to a sensitivity of 0.5 ngg ‐1 of food sample.