Premium
Isolation and characterization of polygalacturonase and invertase from a yeast Endomycopsis fibuligera (3812) bioconversion of date wastes
Author(s) -
ELSAYED SANAA T.,
MOHARIB SORIAL A.,
JWANNY ETIDAL W.
Publication year - 1994
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.1994.tb02067.x
Subject(s) - pectinase , invertase , bioconversion , sucrose , yeast , chemistry , food science , enzyme , biochemistry , fermentation
Properties of polygalacturonase [E.C. 3.2.1.15] (E 1 ) and invertase (E.C. 3.2.1.26] (E 2 ) isolated and purified in powdered form from Endomycopsis fibuligera grown on date wastes were measured. The molecular weights were estimated to be about 32000 and 69500, and they were highly specific for hydrolysing (1–4) and (1–2) glycosidic links respectively. the K m value of E 1 was 7.1 × 10 ‐2 M for soluble‐pectic acid and that for E 2 was 6.7 × 10 ‐2 M for sucrose at pH4.5, and optimal pHs were 3.5 for E 1 and 4.0 for E 2 . the purified enzymes are stable on storage for more than one year at ‐ 20°C.