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Autolysis of Squid Mantle Muscle Protein as Affected by Storage Conditions and Inhibitors
Author(s) -
KONNO KUNIHIKO,
FUKAZAWA CHIZURU
Publication year - 1993
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1993.tb06147.x
Subject(s) - autolysis (biology) , chemistry , myosin , biochemistry , mantle (geology) , pyrophosphate , chromatography , food science , biophysics , enzyme , biology , paleontology
Autolysis of squid mantle muscle was investigated under various conditions using muscle homogenate as a model system. Cleavage of myosin into heavy and light meromyosin was prominent during autolysis. Storage conditions such as NaCl concentration and temperature affected not only the rate of autolysis but also the products formed. Conditions for maximal autolysis were: NaCl concentration 0.3M, pH 7 and temperature 40°C. Autolysis was well inhibited by ethylenediaminetetra acetic acid and Na‐pyrophosphate at <25°C and by phenyhnethylsulfonyl fluoride and chymotrypsin inhibitor extracted from potato tuber at >35°C suggesting two different types of proteinases were involved.