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Lipid Extraction from Channel Catfish Muscle: Comparison of Solvent Systems
Author(s) -
ERICKSON M. C.
Publication year - 1993
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1993.tb03217.x
Subject(s) - dichloromethane , chloroform , chemistry , chromatography , methanol , solvent , extraction (chemistry) , petroleum ether , hexane , phospholipid , catfish , organic chemistry , biochemistry , membrane , fish <actinopterygii> , biology , fishery
During extraction of lipids from catfish minced muscle using 9 solvent systems, phospholipid and triacylglycerol recoveries were similar when tissue was mixed on a vortex (under nitrogen) with about 20 vol of chloroform:methanol (2:1), hexane:isopropanol (3:2), chloroform :isopropanol (7:11), dichloromethane:methanol (2:l) or chloro‐form:methanol:water (2:2:1). Only a few minutes were required to extract a tissue sample by these methods as compared to a few hours for a Soxhlet extraction (petroleum ether) or for extraction by a dry column procedure (dichloromethane:methanol, 9:l). The time available for interaction of the muscle tissue with the dichlorome‐thane:methanol (9:l) system was critical in extraction of lipids, particularly phospholipids.