Agar Medium for Enumeration of Fecal Coliforms | Zendy

CHEN HSINGCHEN | Zendy; WU SHEUDER | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Agar Medium for Enumeration of Fecal Coliforms

Author(s) -

CHEN HSINGCHEN,

WU SHEUDER

Publication year - 1992

Publication title -

journal of food science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 150

eISSN - 1750-3841

pISSN - 0022-1147

DOI - 10.1111/j.1365-2621.1992.tb06882.x

Subject(s) - enumeration , fecal coliform , feces , bromocresol purple , agar , brilliant green , food science , microbiology and biotechnology , biology , most probable number , chemistry , veterinary medicine , chromatography , bacteria , mathematics , medicine , ecology , water quality , genetics , organic chemistry , combinatorics

Spread‐inoculated plates of fecal‐coliform agar (FCA) were pre‐incubated 2 hr at 35°C, and then transferred to 44.5°C for 22 hr. Fecal coliforms on FCA produce acids and CO 2 , which react with bromocresol purple and calcium lactate to form yellow to yellow‐green colonies surrounded by a pale yellow zone. Recovery of control or freeze‐stressed Escherichia coli on FCA was about 1 log cycle lower than that with nonselective media. The accuracy for FCA to enumerate fecal coliforms from 138 typical colonies of three food samples was 98.6%; while the false positive was 1.4%. When the method was compared with the standard 3‐tube MPN method for fecal coliforms, counts from two methods were not significantly different (p<0.05).

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research