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Ascorbic, Dehydroascorbic and Isoascorbic Acid Simultaneous Determinations by Reverse Phase Ion Interaction HPLC
Author(s) -
ZAPATA SERGIO,
DUFOUR JEANPIERRE
Publication year - 1992
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1992.tb05527.x
Subject(s) - dehydroascorbic acid , chemistry , chromatography , derivatization , ascorbic acid , high performance liquid chromatography , cartridge , absorbance , potassium bromide , potassium , counterion , potassium phosphate , methanol , column chromatography , bromide , reproducibility , ion , organic chemistry , mechanical engineering , food science , engineering
Detection was by UV absorbance (after pre‐column derivatization of dehydroascorbic acid with 1,2‐phenylenediamine). When absent, isoascorbic acid could be used as internal standard. Isocratic separation was accomplished in 11 min using the eluent, methanol‐water (5:95, v/v) containing potassium dihydrogen phosphate (50 mM) and the counterion hexadecyltrimethylammonium bromide (5 mM). Sample preparation steps using Sep‐pak C18 cartridge were minimal. Ten ppm could be detected for each compound with good reproducibility (c.v. <2%). The method was used to determine vitamin C content in selected foods and beverages.