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Characteristics and Functional Properties of Protein Isolates from Various Peanut ( Arachis hypogaea L. ) Cultivars
Author(s) -
KIM NAMSOO,
KIM YOUNG JIN,
NAM YOUNG JUNG
Publication year - 1992
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1992.tb05504.x
Subject(s) - arachis hypogaea , cultivar , sephadex , polyacrylamide gel electrophoresis , size exclusion chromatography , storage protein , gel electrophoresis , biology , sodium dodecyl sulfate , chemistry , botany , biochemistry , gene , enzyme
Protein isolates from 9 cultivars of peanut ( Arachis hypogaea L.) were compared. Sephadex G‐200 gel filtration and nondenaturing polyacrylamide gel electrophoresis (NPAGE) of the protein isolates showed very similar patterns of arachin and nonarachin fractions. The content of individual fractions was somewhat different among cultivars, possibly indicating genetic variability. Sodium dodecyl sulfate‐PAGE (SDS‐PAGE) showed that the protein isolates had extremely complex sub‐unit compositions. The content of acidic amino acids of the protein isolates was 34–38%, basic was 15–17%. Differences in some functionalities such as solubility and emulsification activity were conspicuous among cultivars.