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Silica‐based and Polymeric Columns for Reversed‐phase HPLC Analysis of Cheddar Cheese
Author(s) -
LICHAN E.,
KWAN L.,
NAKAI S.,
AMANTEA G.F.
Publication year - 1992
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1992.tb05492.x
Subject(s) - chemistry , chromatography , reagent , trifluoroacetic acid , phosphoric acid , high performance liquid chromatography , divinylbenzene , polystyrene , acetonitrile , copolymer , aqueous solution , elution , reversed phase chromatography , styrene , organic chemistry , polymer
Octadecyl polymeric (ODP) vs octadecyl silica (ODS) reversed‐phase high‐performance liquid chromatography (RP‐HPLC) columns were compared for analytical separation of water‐soluble fractions extracted from Cheddar cheese. A polystyrene divinylbenzene copolymer‐based column did not resolve cheese water‐soluble components. Good resolution was achieved on a vinyl alcohol copolymer ODP column as well as two ODS columns under optimized conditions of gradient elution, using increasing concentrations of aqueous acetonitrile containing ion‐pair reagent. However, the ODP column had longer useful life characteristics. Use of phosphoric instead of trifluoroacetic acid as ion‐pair reagent produced a more stable chromatogram baseline and may prolong column useful life. ODP‐RP‐HPLC could be useful to monitor ripening of Cheddar cheese samples.