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Isolation of α‐Terpineol Dehydratase from Pseudomonas gladioli
Author(s) -
CADWALLADER K.R.,
BRADDOCK R.J.,
PARISH M.E.
Publication year - 1992
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1992.tb05464.x
Subject(s) - terpineol , chemistry , dehydratase , limonene , enzyme , chromatography , pseudomonas , biochemistry , bacteria , biology , essential oil , genetics
An enzyme which converts the citrus by‐product limonene to α‐terpineol was purified 13‐fold. After cell disruption of P. gladioli , α‐terpineol dehydratase (α‐TD) remained associated with a heterogeneous group of particulate material. α‐TD was partially solubilized by extraction with 10 mM HEPES buffer, pH 7.0 containing 2.0% (w/v) Triton X‐100 and 0.5M sodium trichloroacetate. Two soluble forms existed in 1.0% (w/v) Triton X‐100 with apparent molecular weights of 94,500 and 206,500 daltons.