Premium
Maltotetraose Production Using Pseudomonas stutzeri Exo‐α‐Amylase in a Membrane Recycle Bioreactor
Author(s) -
WOO GUNJO,
McCORD J. D.
Publication year - 1991
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1991.tb14631.x
Subject(s) - pseudomonas stutzeri , chemistry , bioreactor , substrate (aquarium) , industrial and production engineering , amylase , chromatography , enzyme , enzyme assay , starch , food science , biochemistry , bacteria , organic chemistry , biology , ecology , genetics , electrical engineering , engineering
For a continuous process using maltotetraose (G 4 )‐forming amylase in a membrane recycle bioreactor (MRB), the enzyme was partially purified to specific activity 21.5 IU/mg protein. The enzyme was stable enough to convert starch into G 4 in batch and MRB systems. Optimum conditions in the MRB using hollow fiber with 1,000 molecular weight cut‐off were: substrate concentration 1.0% (w/v), enzyme concentration 70 IU/L reactant, and residence time 83 min. Productivity at steady state under optimized conditions was 0.99 g/L/hr. Excessive residence time decreased product purity by further conversion of G 4 to lower molecular weight compounds due to endo‐acting activity of the enzyme.