Premium
New Method for Analysis of Trimethylamine Oxide Using Ferrous Sulfate and EDTA
Author(s) -
WEKELL JOHN C.,
BARNETT HAROLD
Publication year - 1991
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1991.tb07993.x
Subject(s) - trimethylamine , ethylenediaminetetraacetic acid , chemistry , ferrous , sulfate , trichloroacetic acid , nuclear chemistry , chloride , extraction (chemistry) , inorganic chemistry , chromatography , chelation , biochemistry , organic chemistry
In the analysis of trimethylamine oxide (TMAO) in fish tissues, an equimolar mixture of ferrous sulfate (FeSO 4 ) and disodium ethylenediaminetetraacetic acid (EDTA) (0.1 M) in acetate buffer (0.8 M, pH 4.5) was found to be a satisfactory replacement for titanium chloride (TiCl 3 ). The TiCl 3 method for TMAO was usually conducted at 80°C; the FeSO 4 ‐EDTA reaction at 50°C appeared complete in 5 min and yielded comparable results. The use of an acetate buffer alleviated any interference from the trichloroacetic acid used for extraction of TMAO and trimethylamine (TMA) from fish tissue.