Premium
Residual Avid in Activity in Cooked Egg White Assayed with Improved Sensitivity
Author(s) -
DURANCE T. D.
Publication year - 1991
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1991.tb05361.x
Subject(s) - avidin , egg white , chemistry , chromatography , extraction (chemistry) , residual , white (mutation) , food science , biochemistry , biotin , mathematics , algorithm , gene
An assay, based on binding of the dye 2–(4'hydroxyazobenzene)‐benzoic acid (HABA) and widely used in studies of avidin in liquid systems, was combined with an aqueous extraction method and used to assay avidin in cooked egg white. The method was validated for linearity of response to increasing avidin concentrations, precision, accuracy, recovery and sensitivity. Mean residual avidin activity in fried, poached and boiled (2 min) egg white was 33, 71 and 40% of the activity in raw egg white.