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Comparison of Rapid Methods for isolation and Enumeration of Clostridium perfringens in Meat
Author(s) -
ALI MOHAMMED S.,
FUNG DANIEL Y. C.,
KASTNER CURTIS L.
Publication year - 1991
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1991.tb05282.x
Subject(s) - clostridium perfringens , petri dish , anaerobic exercise , clostridium , enumeration , microbiology and biotechnology , isolation (microbiology) , food science , inoculation , biology , chemistry , mathematics , bacteria , immunology , combinatorics , physiology , genetics
Three strains of Clostridium pefringens , ATCC 12924, ATCC 12917, and ATCC 14809, were inoculated separately into thioglycollate broth and ground beef samples and enumerated by Fung's Double Tube (FDT), Oxyrase Enzyme (OE), Gas Pak Anaerobic System (GPAS), and Anaerobic Petri Dish (APD) methods. Recovery of the three strains by FDT was significantly higher (P < 0.05) than that by the other three methods from both thioglycollate broth and ground beef, with OE second highest. FDT gave better recovery (84%) in less time (8–10 hr) at 42°C compared with the OE method (61%). FDT was also least costly.