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Continuous Hydrolysis of Milk Protein in a Membrane Reactor
Author(s) -
MANNHEIM A.,
CHERYAN M.
Publication year - 1990
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1990.tb06769.x
Subject(s) - ultrafiltration (renal) , chemistry , membrane reactor , membrane , casein , chromatography , hydrolysis , substrate (aquarium) , permeation , enzyme , chemical engineering , biochemistry , biology , engineering , ecology
Casein was hydrolyzed using Alcalase in a continuous stirred tank membrane reactor (CSTMR) using a hollow fiber ultrafiltration membrane. A design of experiments methodology was used to optimize the four performance variables: enzyme concentration, substrate concentration, permeate flux and reactor volume. High conversion (>90%) of the casein could be achieved after one hour at 50°C and pH 8. Reactor activity was most affected by the enzyme‐to‐substrate ratio. The stability of the reactor (i.e., decay of activity with time) was a function of enzyme leakage through the membrane, thermal inactivation and enzyme‐membrane interactions.