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Simultaneous Determination by HPLC of Fat‐Soluble Vitamins in Albacore (Thunnus alalunga)
Author(s) -
POZO R. G.,
SAITUA E. S.,
UNCILLA I.,
MONTOYA J.A.
Publication year - 1990
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1990.tb06020.x
Subject(s) - albacore , chemistry , saponification , fat soluble vitamin , chromatography , vitamin , thunnus , high performance liquid chromatography , food science , tuna , chloroform , vitamin e , fish <actinopterygii> , biochemistry , biology , antioxidant , fishery
The fat‐soluble vitamins A, D and E were determined by a liquid chromatographic method. The vitamins were extracted from the tissues with chloroform and methanol, saponified and separated on a Lichrosorb normal phase column followed by UV detection. Recoveries of added vitamins in liver samples were 93.0% for vitamin A, 90.0% for vitamin D and 92.1% for vitamin E. The results from a study of fat‐soluble vitamins in samples of albacore showed that the vitamin A and D were found only in fish liver and vitamin E was found in the liver and the edible muscle.