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Lipid Composition and Hydration Characteristics of Lung Protein Isolates Defatted by Several Solvents
Author(s) -
ALCOCER M.J.C.,
ARÊAS J.A.G.
Publication year - 1990
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1990.tb06007.x
Subject(s) - chemistry , solvent , chromatography , composition (language) , polarity (international relations) , polar , fraction (chemistry) , cholesterol , phospholipid , biochemistry , membrane , philosophy , physics , astronomy , cell , linguistics
Different portions of a protein isolate from bovine lungs were exhaustively defatted by solvents of varying polarities. Phospholipids, cholesterol and cholesterol esters, free fatty acids, mono‐, di‐ and triacylglycerols were quantitatively determined in the extracts. Their amount was dependant on the polarity of the solvent employed. Neutral lipids were quantitatively removed by all solvents whereas the amount of phospholipids extracted increased with solvent polarity. The water monolayer capacity of the defatted protein (obtained by the BET equation) showed an increase up to the maximum with the increase of the residual lipid content (determined by Folch wash). The results indicated that more protein polar sites were exposed when the lipid fraction (mainly phospholipids) was present.