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Comparison of Homogenization by Blending or Stomaching on the Recovery of Listeria monocytogenes from Beef Tissues
Author(s) -
DICKSON JAMES S.
Publication year - 1990
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1990.tb05199.x
Subject(s) - listeria monocytogenes , homogenization (climate) , listeria , bacteria , phosphate buffered saline , food science , chemistry , inoculation , microbiology and biotechnology , biology , chromatography , immunology , biodiversity , ecology , genetics
Homogenization by blending or stomaching was compared for the recovery of Listeria monocytogenes from inoculated intact beef tissue. There were no differences in numbers of recovered bacteria (P>0.10) attributable to either homogenization time or method. Fewer viable bacteria (P<0.05) were recovered in phosphate buffer than either buffered peptone water or 2% trisodium citrate buffer. Tween 80 increased the numbers of bacteria recovered from fat tissue (P<0.05). Stomaching is an acceptable method for homogenizing samples for Listeria analysis.