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Lipase Mediated Synthesis of Low Molecular Weight Flavor Esters
Author(s) -
WELSH FRANK W.,
WILLIAMS ROSS E.,
DAWSON KATHRYN H.
Publication year - 1990
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1990.tb03599.x
Subject(s) - pseudomonas fluorescens , rhizomucor miehei , substrate (aquarium) , lipase , chemistry , butyrate , ethyl butyrate , yield (engineering) , hexane , triacylglycerol lipase , organic chemistry , enzyme , chromatography , ethyl acetate , biology , fermentation , bacteria , ecology , genetics , materials science , metallurgy
Screening 27 commercial lipases showed that enzymes from Candida cylindracea, Pseudomonas fluorescens and Mucor miehei (immobilized) promoted synthesis of selected low molecular weight esters in nonaqueous systems. Maximum production after 24 hr incubation was obtained with substrate concentrations of 0.05 mol/L for isopentyl acetate, 0.2 mol/L for ethyl butyrate and 0.3 mol/L for isopentyl butyrate. Yield of butyl butryate was almost 100% at acid substrate greater than 0.2 mol/L. Substrate inhibition was observed with P. fluorescens lipase but not with C. cylindracea or M. miehei lipases, up to 1 mol/L. Hexane, octane and decane could be used as reaction media except for ethyl butyrate synthesis where hexane was the medium of choice. Poor synthesis was achieved when methylene chloride was used.