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Respiratory Enzyme Activity in Low Temperature Sweetening of Susceptible and Resistant Potatoes
Author(s) -
BARICHELLO VALERIE,
YADA RICKEY Y.,
COFFIN ROBERT H.,
STANLEY DAVID W.
Publication year - 1990
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1990.tb01597.x
Subject(s) - cultivar , sucrose , respiration , fructose , dehydrogenase , sweetening , chemistry , phosphofructokinase , respiration rate , food science , horticulture , carbohydrate , biology , botany , enzyme , biochemistry , glycolysis , sweetening agents
During storage at 4°C and 12°C, a potato cultivar susceptible to chill‐sweetening (Norchip) accumulated significantly (P≤0.05) higher levels of sucrose, fructose and glucose than a potato selection resistant to chill‐sweetening (ND 860‐2). ND 860‐2 tubers exhibited a significantly (P≤0.05) higher respiration rate throughout storage, reflected in higher activities of phosphofructokinase (PFK), glucose‐6‐phos‐phate dehydrogenase (G6PDH) and 6‐phosphogluconate dehydrogenase (6PGDH). Storage significantly (P≤0.05) reduced respiration rate for both cultivars. G6PDH showed no significant (P>0.05) difference in specific activity or V max between 4°C and 12°C for either cultivar. However, Km decreased at 4°C for both cultivars, possibly due to build up of substrate.