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An enzyme immunoassay for nisin
Author(s) -
FALAHEE M. B.,
ADAMS M. R.,
DALE J. W.,
MORRIS B. A.
Publication year - 1990
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.1990.tb01119.x
Subject(s) - bioassay , nisin , polyclonal antibodies , antiserum , immunoassay , chromatography , detection limit , enzyme , chemistry , microbiology and biotechnology , biology , biochemistry , antibody , immunology , genetics , antimicrobial
Summary The development of an enzyme‐linked immunosorbent assay (ELISA) for nisin using polyclonal antiserum is described. The method has a limit of detection of 1.9 X 10 −2 i.u. ml −1 and correlates well with the bioassay. Its use in the analysis of commercial processed cheese samples is described and the results obtained compared with those from the bioassay.