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Total Sterols in Seafoods: Iatroscan TLC/FID Versus the Kovacs GLC/FID Method
Author(s) -
WALTON C. G.,
RATNAYAKE W. M. N.,
ACKMAN R. G.
Publication year - 1989
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1989.tb07884.x
Subject(s) - saponification , sterol , chromatography , chemistry , gas chromatography , fish <actinopterygii> , cholesterol , biology , fishery , biochemistry
THE TOTAL STEROLS of selected seafood samples were determined, after saponification, by latroscan TLC/FID and the results were compared with those obtained by the more common technique of gas‐liquid chromatography which gives individual sterols. No significant difference was found between the techniques in terms of quantitative accuracy for total sterols. In many products the sterol compositions were known or specific compositional details were not important. Thus, in most finfish and Crustacea species, cholesterol constituted over 90% of the sterols and, therefore, the Chromarodlatroscan TLC/FID with or without inclusion of an internal standard would give an accurate estimation of the cholesterol in fish products.