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Soy Proteins in Milk Replacers Identified by Immunoblotting
Author(s) -
VENTLING B.L.,
HURLEY W.L.
Publication year - 1989
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1989.tb04705.x
Subject(s) - electroblotting , soy protein , polyacrylamide gel electrophoresis , chemistry , vegetable proteins , food science , nitrocellulose , gel electrophoresis , chromatography , biochemistry , enzyme , membrane
A method was developed for detecting soy proteins in milk replacers and infant formulas. The method is based upon polyacrylamide gel electrophoresis of proteins, electroblotting the proteins to nitrocellulose, and detection of specific proteins by immunoblotting. As little as 25 ng of total soy protein per gel lane can be detected with this method. Proteins from other plant products were detected by the method but care must be used in comparing milk replacer proteins directly to soy proteins. This method should be of value to the animal and food industries that manufacture and use milk replacers.