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Effect of Various Agents Upon the Attachment of Pseudomonas fragi to Stainless Steel
Author(s) -
HERALD PAULA J.,
ZOTTOLA EDMUND A.
Publication year - 1989
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1989.tb03106.x
Subject(s) - concanavalin a , sodium periodate , chemistry , sodium , sodium dodecyl sulfate , trypsin , periodate , nuclear chemistry , pseudomonas , enzyme , chromatography , biochemistry , in vitro , bacteria , organic chemistry , biology , genetics
Epifluorescent microscopy was used to assay for the effect of selected chemicals and enzymes upon physical attachment of Pseudomonas fragi to stainless steel. The assays were conducted after the pretreatment of cells with the agents, in the presence of agents, and in the removal of attached cells with test agents. Compared to the controls, sodium periodate, cetyltrimethylammonium bromide (Cetavlon), NaOH, and Concanavalin A inhibited the attachment process in the pretreatment and presence assays. Trypsin, sodium dodecyl sulfate (SDS), NaOH and sodium periodate were effective in removing attached cells, while Cetavlon and Triton X‐100 stabilized the attachment of cells to the stainless steel surface and prevented their removal. Neither EDTA nor mechanical removal of flagella had a significant effect upon attachment. These results suggested that attachment of P. fragi to stainless steel involved a polysaccharide and protein matrix surrounding the cells.