Premium
An Improved Enzyme Immunoassay for Limonin
Author(s) -
RAM BHANU P.,
JANG LARRY,
MARTINS LORELEI,
SINGH PRITHIPAL
Publication year - 1988
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1988.tb10245.x
Subject(s) - limonin , immunoassay , ovalbumin , chemistry , chromatography , polyvinyl alcohol , antibody , enzyme , biochemistry , immunology , antigen , medicine , organic chemistry
Performance of the previously reported enzyme immunoassay for determination of limonin in citrus juice was improved by stabilization of anti‐limonin‐antibody‐coated microtitration wells with 1% solution of ovalbumin and polyvinyl alcohol. Standard curves of the antibody stabilized wells showed insignificant change upon storage at 4°C for 5 months. Average inter‐ and intra‐assay coefficients of variation were 6.5% and 3.5%, respectively, assuring consistent assay performance.