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Application of Response Surface Methodology (RSM) to the In Vitro Measurement of Bile Salt Binding Under Physiological Conditions
Author(s) -
CADDEN ANNMARIE
Publication year - 1988
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1988.tb08993.x
Subject(s) - bran , salt (chemistry) , incubation , chemistry , response surface methodology , phosphate buffered saline , phosphate , incubation period , chromatography , in vitro , particle size , sodium , food science , buffer (optical fiber) , biochemistry , raw material , telecommunications , organic chemistry , computer science
Response surface methodology was used to assess the effects of adjusting incubation time, sample weight and buffer pH on the in vitro measurements of sodium glycocholate binding by ground and un‐ground AACC wheat bran. The degree of bile salt binding could be increased by increasing incubation time and by reducing buffer pH. Maximum binding occurred if the sample weight per incubation were approximately 200 mg. Reducing particle size slightly modified the effects of time and pH. Washing the wheat bran fibers with phosphate buffer reduced the total quantity of bile salts bound; however, response surface trends were not affected.