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Detection of Singlet Oxygen Generation by Chlorophyll using Electron Paramagnetic Resonance Spectroscopy
Author(s) -
WHANG K.,
PENG I. C.
Publication year - 1988
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1988.tb07880.x
Subject(s) - electron paramagnetic resonance , chemistry , singlet oxygen , butylated hydroxyanisole , photochemistry , singlet state , spectroscopy , oxygen , radical , scavenger , analytical chemistry (journal) , nuclear magnetic resonance , organic chemistry , excited state , antioxidant , physics , quantum mechanics , nuclear physics
Electron paramagnetic resonance (EPR) spectroscopy was used to detect the photosensitized generation of singlet oxygen ( 1 O 2 ) by chlorophyll b. Illumination of a chlorophyll b solution containing a spin trap (2,2,6,6‐tetramethyl‐4‐piperidone, TMPD) yielded pronounced EPR signals for TAN (2,2,6,6‐tetramethyl‐4‐piperidone‐N‐oxyl) suggesting the presence of 1 O 2 . N,N,N′,N′‐tetramethylethylene diamine (TMEDA, a 1 O 2 quencher) and a combination of TMEDA with a free radical scavenger (butylated hydroxyanisole, BHA) completely eliminated the TAN signal. BHA alone did not exert this inhibitory effect.