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Chiral Liquid Chromatography for Resolving Malic Acid Enantiomers in Adulterated Apple Juice
Author(s) -
DONER L. W.,
CAVENDER P. J.
Publication year - 1988
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1988.tb07870.x
Subject(s) - malic acid , high performance liquid chromatography , chemistry , chromatography , enantiomer , valine , malate dehydrogenase , malic enzyme , amino acid , biochemistry , organic chemistry , enzyme , citric acid , dehydrogenase
Reversed‐phase HPLC with an aqueous mobile phase containing the chiral ligand‐exchanger Cu 11 / (N,N‐dimethyl‐L‐valine) 2 resolved the enantiomeric a‐hydroxy acids, D‐ and L‐malate. Post‐column detection with acidic Fe 111 resulted in specific detection of α‐hydroxy acids, so filtered apple juice gave a simple profile. D‐Malic acid in apple juices suspected of being adulterated with synthetic DL‐malic acid is presently determined from the difference between DL‐malic acid (HPLC assay) and L‐malic acid (L‐malate dehydrogenase assay). The potential of the chiral HPLC method relative to the indirect method was evaluated and additional possibilities for direct and more sensitive determination of D‐malate were suggested.