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High Pressure Liquid Chromatography Determination of Histamine in Fish
Author(s) -
GOUYGOU J. P.,
SINQUIN C.,
DURAND P.
Publication year - 1987
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1987.tb14244.x
Subject(s) - chromatography , chemistry , derivatization , trichloroacetic acid , fluorophore , histamine , elution , acetonitrile , extraction (chemistry) , fluorescence , high performance liquid chromatography , medicine , physics , quantum mechanics
Histamine was determined by reversed‐phase high‐pressure liquid chromatography in trichloroacetic acid extracts after derivatization with o‐phthaladehyde. Fluorescence was monitored at 350 nm excitation and 450 nm emission wavelength after elution with water containing 40% acetonitrile. The advantage of the described method is a rapid analysis in an automated system, where no selective extraction procedure is necessary and interfering substances are easily separated from the histamine fluorophore.