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In Vitro Metabolism of Aflatoxin B 1 with Microsomal Enzymes in the Presence of Selected Nutrients
Author(s) -
WHEELER J. L.,
HARRISON M.A.,
KOEHLER P. E.
Publication year - 1987
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1987.tb14101.x
Subject(s) - aflatoxin , chemistry , metabolism , pyridoxine hydrochloride , microsome , ascorbic acid , pyridoxine , selenium , enzyme , biochemistry , vitamin , nutrient , tocopherol , in vitro , chromatography , food science , vitamin e , antioxidant , organic chemistry
High pressure liquid chromatography was used to evaluate the effects of several naturally occurring food components (selenium, vitamins A, E, B 6 and C) on the in vitro metabolism of aflatoxin B 1 (AFB 1 ). AF 1 was incubated with a liver microsomal enzyme metabolizing system with varying concentrations of each nutrient. The following nutrients and levels either inhibited or reduced the metabolism of AFB 1 : sodium selenite (25 μg/mL), d‐ α‐tocopherol (25, 250 and 2500 μg/mL), pyridoxine hydrochloride (2.5 μg/mL), L‐ascorbic acid (25 and 2500 μg/mL) and mixture containing 500 μg/mL of each chemical. Retinol acetate at levels of 2.5 and 25 μg/mL increased the level of AFB 1 metabolized.