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Canola Phytase: Isolation and Characterization
Author(s) -
KIM H.,
ESKIN N.A.M.
Publication year - 1987
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1987.tb14080.x
Subject(s) - phytase , canola , sephadex , size exclusion chromatography , chemistry , isozyme , chromatography , column chromatography , fractionation , biochemistry , enzyme , food science
Two phytase isoenzymes were isolated from 8‐day germinated canola cv Regent. Gel filtration chromatography of an ammonium sulfate fractionated extract on Sephadex G‐100 produced one peak with phytase activity. The phytase fraction was separated into two isoenzymes by DEAE‐cellulose chromatography. The optimum pH was 4.5–5.0 and 5.0 for the phytase isoenzymes 1 and 2, respectively. Both isoenzymes exhibited maximum activity at 50°C. Km values at pH 5.0 were 0.36 and 0.25 mM for phytase 1 and 2 isoenzymes, respectively, while molecular weight determination showed both fraction were identical with a molecular weight of 70,100 ± 4,000 daltons.